The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.
Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.
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MESH: Adenoviridae; MESH: Base Sequence; MESH: Cross-Linking Reagents; MESH: DNA, Single-Stranded; MESH: DNA-Binding Proteins; MESH: Escherichia coli; MESH: Kinetics; MESH: Molecular Sequence Data; MESH: Molecular Weight; MESH: Oligodeoxyribonucleotides; MESH: Plasmids; MESH: Polypyrimidine Tract-Binding Protein; MESH: Promoter Regions (Genetics); MESH: RNA-Binding Proteins; MESH: Recombinant Fusion Proteins; MESH: Ultraviolet Rays; MESH: Binding, Competitive; [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
ISSN: 0305-1048; EISSN: 1362-4962; Nucleic Acids Research; https://hal.archives-ouvertes.fr/hal-00277601; Nucleic Acids Research, Oxford University Press, 1996, 24 (9), pp.1608-15. ⟨10.1093/nar/24.9.1608⟩